Key components of the fission machinery are interchangeable

作者：Yang Jia Shu; Zhang Leiliang; Lee Stella Y; Gad Helge; Luini Alberto; Hsu Victor W^*

来源：Nature Cell Biology, 2006, 8(12): 1376-U34.

DOI：10.1038/ncb1503

Brefeldin- A ADP- ribosylated substrate (BARS) and dynamin function in membrane fission in distinct intracellular transport pathways(1), but whether their functions are mechanistically similar is unclear. Here, we show that ARFGAP1, a GTPase-activating protein (GAP) for ADP- ribosylation factor 1 (ARF1)(2), couples to either BARS or endophilin B for vesicle formation by the coat protein I (COPI) complex- a finding that reveals an unanticipated mechanistic flexibility in mammalian COPI transport. Because dynamin is coupled to endophilin A in vesicle formation by the clathrin- coat complex(3-5), our finding also predicts that dynamin and ARF GAPs are likely to be functional counterparts in membrane fission among different transport pathways that connect intracellular membrane compartments.

• 出版日期2006-12