A novel aggregation-induced emission fluorescent probe for nucleic acid detection and its applications in cell imaging

作者：Xu, Xiaowei; Yan, Shengyong; Zhou, Yimin; Huang, Rong; Chen, Yuqi; Wang, Jiaqi; Weng, Xiaocheng; Zhou, Xiang^*

来源：Bioorganic & Medicinal Chemistry Letters, 2014, 24(7): 1654-1656.

DOI：10.1016/j.bmcl.2014.02.071

• Fluorescent
• Nucleic acid
• Aggregation-induced emission
• Gel electrophoresis
• Cell imaging
• Dye

A new kind of aggregation-induced emission compound was synthesized and used as the probe of nucleic acid. The characterization of this compound was studied. Both the RNA and DNA were detected by using this probe. And the detection scope of DNA and RNA was different. We researched the selectivity of our probe in double and single strand DNA sequences. The visualization of gel electrophoresis and the cell nucleus imaging were researched as well. Compared with the traditional nucleus dye Hoechst 33258, our probe also has the potential to be nucleus dye. And the cell toxicity was well performed by MTT assays.

• 出版日期2014-4-1
• 单位武汉大学