A modeling and simulation perspective on the mechanism and function of respiratory complex I

作者：Haapanen Outi; Sharma Vivek^*

来源：Biochimica et Biophysica Acta-Bioenergetics, 2018, 1859(7): 510-523.

DOI：10.1016/j.bbabio.2018.04.001

• Cellular respiration
• Electron transfer
• Proton pumping
• Mitochondrial dysfunction
• Reactive oxygen species

Respiratory complex I is a giant redox-driven proton pump, and central to energy production in mitochondria and bacteria. It catalyses the reduction of quinone to quinol, and converts the free energy released into the endergonic proton translocation across the membrane. The proton pumping sets up the proton electrochemical gradient, which propels the synthesis of ATP. Despite the availability of extensive biochemical, biophysical and structural data on complex I, the mechanism of coupling between the electron and proton transfer reactions remain uncertain. In this work, we discuss current state-of-the-art in the field with particular emphasis on the molecular mechanism of respiratory complex I, as deduced from computational modeling and simulation approaches, but in strong alliance with the experimental data. This leads to novel synthesis of mechanistic ideas on a highly complex enzyme of the electron transport chain that has been associated with a number of mitochondrial and neurodegenerative disorders.

• 出版日期2018-7