Novel multistep BRET-FRET energy transfer using nanoconjugates of firefly proteins, quantum dots, and red fluorescent proteins

作者:Alam Rabeka; Zylstra Joshua; Fontaine Danielle M; Branchini Bruce R; Maye Mathew M*
来源:Nanoscale, 2013, 5(12): 5303-5306.
DOI:10.1039/c3nr01842c

摘要

Sequential bioluminescence resonance energy transfer (BRET) and fluorescence resonance energy transfer (FRET) from firefly luciferase to red fluorescent proteins using quantumdot or rod acceptor/donor linkers is described. The effect of morphology and tuned optical properties on the efficiency of this unique BRET-FRET system was evaluated.

  • 出版日期2013