A novel aphid resistance locus in cowpea identified by combining SSR and SNP markers

作者：Kusi Francis; Padi Francis K; Obeng Ofori Daniel; Asante Stephen K; Agyare Richard Y; Sugri Issah; Timko Michael P; Koebner Robert; Bao Lam Huynh; Santos Jansen R P; Close Timothy J; Roberts Philip A^*

来源：Plant Breeding, 2018, 137(2): 203-209.

DOI：10.1111/pbr.12563

• Aphis craccivora
• backcrossing
• cowpea
• marker-assisted selection
• polymorphism
• Vigna unguiculata

The utility of combining simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) marker genotyping was determined for genetically mapping a novel aphid (Aphis craccivora) resistance locus in cowpea breeding line SARC 1-57-2 and for introgressing the resistance into elite cultivars by marker-assisted backcrossing (MABC). The locus was tagged with codominant SSR marker CP 171F/172R with a recombination fraction of 5.91% in an F-2 population from Apagbaala' x SARC 1-57-2. A SNP-genotyped biparental recombinant inbred line population was genotyped for CP 171F/172R, which was mapped to position 11.5 cM on linkage group (LG) 10 (physical position 30.514Mb on chromosome Vu10). Using CP 171F/172R for foreground selection and a KASP-SNP-based marker panel for background selection in MABC, the resistance from SARC 1-57-2 was introduced into elite susceptible cultivar Zaayura'. Five BC4F3 lines of improved Zaayura' that were isogenic except for the resistance locus region had phenotypes similar to SARC 1-57-2. This study identified a novel aphid resistance locus and demonstrated the effectiveness of integrating SSR and SNP markers for trait mapping and marker-assisted breeding.

• 出版日期2018-4