Using sensitive surface plasmon resonance to detect binding of peptide molecules and immobilized vancomycin

作者：He Yong-hui; Liu Bin; Zhan Yan-feng; Liu You-nian; Xiang Juan^*

来源：Journal of Central South University of Technology, 2011, 18(4): 1024-1028.

DOI：10.1007/s11771-011-0798-z

• flow injection
• surface plasmon resonance
• vancomycin
• Lys-Lys-Gly-D-Ala-D-Ala
• binding
• sensitivity

To perform the mechanism study of special association for vancomycin and D-Ala-D-Ala-containing peptides on the interface of solution and self-assemble monolayer, the binding between vancomycin and pentapeptide (Lys-Lys-Gly-D-Ala-D-Ala) was investigated by flow injection surface plasmon resonance (FI-SPR) and flow injection quartz crystal microbalance (FI-QCM). To facilitate the formation of a compact vancomycin adsorbates layer with a uniform surface orientation, vancomycin molecules were attached onto a preformed alkanethiol self-assembled monolayer. By optimizing the conditions for the binding between Lys-Lys-Gly-D-Ala-D-Ala and vancomycin on the assembled chip, the detecting limit of Lys-Lys-Gly-D-Ala-D-Ala was greatly improved (reaching 0.5x10(-6) mol/L or 7.5x10(-12) mol). The equilibrium constant of the association of Lys-Lys-Gly-D-Ala-D-Ala with vancomycin was also obtained (K (Ads)=5.0x10(4) L/mol).

• 出版日期2011-8
• 单位中南大学