An affinity chromatography method used to purify His-tag-displaying bio-nanocapsules

作者：Nishimura Yuya; Takeda Koichi; Ishii Jun; Ogino Chiaki; Kondo Akihiko^*

来源：Journal of Virological Methods, 2013, 189(2): 393-396.

DOI：10.1016/j.jviromet.2013.03.008

• Hepatitis B virus
• Bio-nanocapsule
• His-tag
• Affinity chromatography

A bio-nanocapsule derived from the hepatitis B virus (HBV) is expected to be useful as a drug delivery system carrier. Because various types of bio-nanocapsules have been developed, a simple and versatile purification method for bio-nanocapsules would be useful. Therefore, this study was focused on establishing a simple purification method using affinity chromatography by inserting a histidine tag (His-tag) into a bio-nanocapsule. The method achieved a simple, one-step purification with a yield that was 2.5-fold higher than conventional ultracentrifugation, and thus would be a desirable alternative method for recombinant virus-like particle purification.

• 出版日期2013-5