Conversion of shrimp heads to alpha-glucosidase inhibitors via co-culture of Bacillus mycoides TKU040 and Rhizobium sp TKU041

作者：Hsu Chia Hau; Van Bon Nguyen; Anh Dzung Nguyen; Wang San Lang^*

来源：Research on Chemical Intermediates, 2018, 44(8): 4597-4607.

DOI：10.1007/s11164-018-3266-7

• alpha-Glucosidase
• Diabetes
• Bacillus mycoides
• Rhizobium
• Chitin
• Shrimp

Alpha-glucosidase inhibitors (aGIs) have potential use as antidiabetic drugs for the treatment of type II diabetes. Most aGIs place a burden on the liver and cause gastrointestinal distress, therefore the development of new aGIs has become very important. In this study, we investigated the production of aGIs by the co-culture of Bacillus mycoides TKU040 and Rhizobium sp. TKU041 using shrimp head powder (SHP) as the sole source of carbon and nitrogen. After fermentation in 50 mL of 1% SHP-containing medium (0.1% K2HPO4 and 0.05% MgSO4 center dot 7H(2)O, pH 9.2) at 37 degrees C for 4 days, the maximum productivity of aGIs (143 U/mL) was reached. The IC50 of the aGIs produced in the culture supernatant was 3 mg/mL. The aGI activity was only 60% after treatment at pH 3 for 30 min; this increased to 140% after treatment at pH 11 for 30 min. The aGI activity remained at 60% after treatment at 60 degrees C for 30 min.

• 出版日期2018-8