A thiourea-appended rhodamine chemodosimeter for mercury(II) and its bioimaging application

作者:Tantipanja**** Ajcharapan; Prabpai Samran; Suksen Kanoknetr; Kongsaeree Palangpon*
来源:Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2018, 192: 101-107.
DOI:10.1016/j.saa.2017.10.057

摘要

A rhodamine-thiourea conjugate RTP with an o-phenylenediamine linker was developed as a fluorogenic chemodosimeter for Hg2+ detection. In the presence of Hg2+, a colorless solution of RTP turned pink with a maximum absorption band at 555 nm and with a 62-fold fluorescence enhancement at 578 nm (Phi = 0.34). RTP is highly selective to Hg2+ among other metal ions with a detection limit of 1.6 nM (0.3 ppb). A similar rhodamine analog with a flexible ethylenediamine spacer was less selective and less sensitive than RTP. Hg2+ induced cyclic guanylation to yield a benzimidazole moiety and a subsequent ring-opening of the spirolactam unit resulted in chromogenic and fluorogenic changes. The membrane-permeable RTP probe was successfully demonstrated in monitoring of Hg2+ in cultured HeLa cells.

  • 出版日期2018-3-5