Alteration of mitochondrial oxidative phosphorylation in aged skeletal muscle involves modification of adenine nucleotide translocator

作者：Gouspillou Gilles; Bourdel Marchasson Isabelle; Rouland Richard; Calmettes Guillaume; Franconi Jean Michel; Deschodt Arsac Veronique; Diolez Philippe^*

来源：Biochimica et Biophysica Acta-Bioenergetics, 2010, 1797(2): 143-151.

DOI：10.1016/j.bbabio.2009.09.004

• Mitochondrial oxidative phosphorylation
• Top-down control analysis
• Skeletal muscle
• Adenine nucleotide translocator
• Aging

The process of skeletal muscle aging is characterized by a progressive loss of muscle mass and functionality. The underlying mechanisms are highly complex and remain unclear. This study was designed to further investigate the consequences of aging on mitochondrial oxidative phosphorylation in rat gastrocnemius muscle, by comparing young (6 months) and aged (21 months) rats. Maximal oxidative phosphorylation capacity was clearly reduced in older rats, while mitochondrial efficiency was unaffected. Inner membrane properties were unaffected in aged rats since proton leak kinetics were identical to young rats. Application of top-down control analysis revealed a dysfunction of the phosphorylation module in older rats, responsible for a dysregulation of oxidative phosphorylation under low activities close to in vivo ATP turnover. This dysregulation is responsible for an impaired mitochondrial response toward changes in cellular ATP demand, leading to a decreased membrane potential which may in turn affect ROS production and ion homeostasis. Based on our data, we propose that modification of ANT properties with aging could partly explain these mitochondrial dysfunctions.

• 出版日期2010-2