A New Route Toward IPN Photocrosslinked Alginate Hydrogels with Biodegradation Rates, Excellent Biocompatibility and Mechanical Properties

作者：Hago Eltjani Eltahir^*; Li Xinsong; Kai Ji Jin

来源：Journal of Biomaterials and Tissue Engineering, 2014, 4(2): 89-98.

DOI：10.1166/jbt.2014.1149

• Alginate
• Photocrosslinked
• Biodegradable
• Cytocompatibility
• Hydrogel

The photocrosslinked and biodegradable alginate hydrogels were engineered for biomedical applications. IPN photocrosslinkable alginate macromeres were prepared by reacting sodium alginate with N-(3-Aminopropyl) methacrylamide hydrochloride in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride, N-hydroxysuccinimide, gelatin (GE) and transglutaminase enzyme (mTG). Methacrylated alginates were photocrosslinked using ultraviolet light with 0.05% photoinitiator. The effects of photocrosslinking between modified GE-AM blend on mechanical, and biological properties were investigated. The swelling behavior, elastic moduli, and degradation rates of the IPN photocrosslinked alginate hydrogels were quantified and could be controlled by varying the degree of alginate methacrylation. The methacrylated alginate macromere and IPN photocrosslinked alginate hydrogels exhibited no cytotoxic effects and excellent cytocompatibility. Fibroblasts L929 cells were seeded on the surfaces of the cross-linked IPN GE-AM hydrogels and were found to attach firmly by expressing philopodial extensions. The MTT assay confirmed that the cells readily proliferated on the cross-linked IPN GE-AM hydrogels. The fibroblast cell-matrix interaction demonstrated that the active biocompatibility of the hydrogels was facilitated by using GE and cross-linking. In conclusion, our results suggest that cross-linked IPN GE-AM hydrogels hold promise for tissue engineering applications.

• 出版日期2014-2
• 单位东南大学