Promoter regions of potato vacuolar invertase gene in response to sugars and hormones

作者：Ou, Yongbin; Song, Botao; Liu, Xun; Xie, Conghua; Li, Meng; Lin, Yuan; Zhang, Huiling; Liu, Jun^*

来源：Plant Physiology and Biochemistry, 2013, 69: 9-16.

DOI：10.1016/j.plaphy.2013.04.015

• Cis-acting element
• Potato
• StvacINV1 promoter
• Transcriptional regulation

Potato vacuolar acid invertase (StvacINV1) (beta-fructofuranosidase; EC 3.2.1.26) has been confirmed to play an important role in cold-induced sweetening of potato tubers. However, the transcriptional regulation mechanisms of StvacINV1 are largely unknown. In this study, the 5'-flanking sequence of StvacINV1 was cloned and the cis-acting elements were predicted. Histochemical assay showed that the StvacINV1 promoter governed beta-glucuronidase (GUS) expression in potato leaves, stems, roots and tubers. Quantitative analysis of GUS expression suggested that the activity of StvacINV1 promoter was suppressed by sucrose, glucose, fructose, and cold, while enhanced by indole-3-acetic acid (IAA), and gibberellic acid (GA(3)). Further deletion analysis clarified that the promoter regions from -118 to -551, -551 to -1021, and -1021 to -1521 were required for responding to sucrose/glucose, GA(3), and IAA, respectively. These findings provide essential information regarding transcriptional regulation mechanisms of StvacINV1.

• 出版日期2013-8
• 单位华中农业大学