MT1-MMP on the cell surface causes focal degradation of gelatin films

作者：d'Ortho MP; Stanton H; Butler M; Atkinson SJ; Murphy G; Hembry RM^*

来源：FEBS Letters, 1998, 421(2): 159-164.

DOI：10.1016/S0014-5793(97)01555-X

• matrix metalloproteinase
• membrane-type matrix metalloproteinase
• gelatinase A
• matrix degradation
• confocal microscopy

The membrane-type matrix metalloproteinases (MT-MMPs) are a subclass of the matrix metalloproteinase (MMP) family which uniquely possess a C-terminal transmembrane domain and are initiators of an activation cascade for progelatinase A (MMP-2), Recent studies have shown that they can also efficiently directly degrade a number of matrix macromolecules, We now show that cells expressing MT1-MMP on their cell surfaces cause subjacent proteolysis of a gelatin film and that this proteolysis is inhibited by TIMP-2 but not by TIMP-1. These data indicate that expression of MT1-MMP on the cell surface may lead to both progelatinase A activation and extracellular matrix degradation.

• 出版日期1998-1-9