alpha-ENaC in bullfrog embryo: expression in cement gland, gills and skin

作者：Fujimaki Aoba Kayo; Tanaka Kayoko; Inomata Reiko; Jensik Philip J; Takada Makoto^*

来源：Cell and Tissue Research, 2014, 355(1): 103-109.

DOI：10.1007/s00441-013-1724-5

• Cement gland
• ENaC
• Bullfrog
• Anuran embryo
• Frog skin

The epithelial sodium channel (ENaC) is involved in Na+ responses such as Na+ absorption and salt taste. The alpha ENaC subunit (alpha-ENaC) is expressed in the skin of both the adult and larval (tadpole) bullfrog. alpha-ENaC expression in the developing bullfrog embryo has not been previously investigated. In this study, the expression of alpha-ENaC at various stages (Sts.) of bullfrog embryonic development is assessed by western blot and immunofluorescence analysis. Bullfrog alpha-ENaC (alpha-fENaC) protein was detected by western blot in embryos at Sts. (Gosner/Shumway) 19, 21 and 25. Immunofluorescence studies indicate that alpha-fENaC was localized to the embryonic cement glands at St. 18 (muscular response), St. 19 (heart beat) and St. 21 (mouth open and/or cornea transparent), to the external gills at St. 21 and to the outermost cell-layer of the skin at St. 25 (operculum complete). The s) of ENaC in these embryonic structures remain to be elucidated.

• 出版日期2014-1