Short Term Cytokine Change of Bone Marrow Derived Mononuclear Cell

作者：Song Gun Sang; Noh Kyung Hwan; Moon Young Lae^*; You Jae Won; Song Chang Hoon

来源：Tissue Engineering and Regenerative Medicine, 2008, 5(4-6): 546-551.

• Bone marrow
• cytokine
• RANTES
• IP-10
• IL-12(p40)

Cytokines involve in both inflammatory and healing mechanism. The aim of this study to evaluate the changing nature of cytokines and thereby appropriate time for using bone marrow derived monuclear cells(BM-MNC) to acquire best effect. We chose 8 patients whose age is ranged from 38 to 51(mean=49). Bone marrow aspirate was obtained from iliac bone of patients with consented to the procedure. Cytokines from plasma layer acquired after 30 minutes of centrifugation at 1,820rpm were analyzed. Cytokines were evaluated after abstraction of mononuclear cells using ficoll method. The cytokines evaluated were Eotaxin, GM-CSF, IFN r, IL-1 a, IL-1 bIL-10, IL-12(p40), IL-12(p70),IL-13, IL-15, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IP-10, MCP-1, MIP-1a, RANTES and TNF-a. High level of IP-10, IL-12 p40, RANTES were detected after centrifugation. Additionally, high level of IL-6, IL-7, IL-8, MCP-1, MIP-1a were noticed in 1 and 7 days following culture. Mononuclear cells derived from bone marrow have high activity character and the change of cytokine was observed after simple culture. Our results show that bone marrow derived mononuclear cells immediately after centrifugation of bone marrow is most effective.

• 出版日期2008-12