Augmentation for Cell Spreading and Migration by A Soluble Fraction of Cotton-Derived Carboxymethyl Cellulose

作者：Aoshima Motonori^*; Jo Yoshio

来源：Kobunshi Ronbunshu, 2013, 70(6): 273-281.

• Carboxymethyl Cellulose
• Soluble Hemostatic Material
• Cell Spreading
• Cell Migration
• Wound Healing

We report that a soluble fraction of carboxymethyl cellulose (Sol-CM) stimulates the cell adhesion, cell spreading, and cell migration of three types of cells. Sol-CM facilitates cell spreading and cell migration of NIH-3T3 mouse fibroblasts on acidic and neutral proteins, including bovine serum albumin. A synthetic Arg-Gly-Asp-Ser (RGD-containing)-peptide completely inhibited cell spreading, but not Arg-Gly-Glu-Ser (RGE-containing)-peptide. The effect of cell spreading depends on the molecular mass of the Sol-CM. Sol-CM can be broken down by either electron beam irradiation or acid treatment, and we found that the cell spreading effect decreased remarkably with decreasing molecular mass. Molecular masses of less than 140 kDa show no effect for cell spreading, indicating that a certain molecular length of Sol-CM is required for augmentation of cell spreading. Furthermore, the effect of Sol-CM on three types of cells, NIH-3T3 mouse fibroblast, Detroit 562 human epithelial cell and 293 human epithelial cell was studied. Sol-CM did not show any effect on 293 human epithelial cells that do not express integrin beta(3)-subunits onto their cell surface. We found that the following factor contribute to the Sol-CM effect: beta(1-4)-linked glycosidic bonds in the polysaccharide, negatively charged carboxymethyl groups, a molecular length of more than 300 nm, a coating protein which has a weakly acidic or neutral isoelectric point, and integrin beta(3)-subunits onto the cell surface.

• 出版日期2013