A load driver device for engineering modularity in biological networks

作者：Mishra Deepak; Rivera Phillip M; Lin Allen; Del Vecchio Domitilla; Weiss Ron^*

来源：Nature Biotechnology, 2014, 32(12): 1268-U141.

DOI：10.1038/nbt.3044

The behavior of gene modules in complex synthetic circuits is often unpredictable(1-4). After joining modules to create a circuit, downstream elements (such as binding sites for a regulatory protein) apply a load to upstream modules that can negatively affect circuit 1,5). Here we devised a genetic device named a load driver that mitigates the impact of load on circuit function, and we demonstrate its behavior in Saccharomyces cerevisiae. The load driver implements the design principle of timescale separation: inclusion of the load driver's fast phosphotransfer processes restores the capability of a slower transcriptional circuit to respond to time-varying input signals even in the presence of substantial load. Without the load driver, we observed circuit behavior that suffered from a 76% delay in response time and a 25% decrease in system bandwidth due to load. With the addition of a load driver, circuit performance was almost completely restored. Load drivers will serve as fundamental building blocks in the creation of complex, higher-level genetic circuits.

• 出版日期2014-12