An In Vitro and In Vivo Study of the alpha-Amylase Activity of Phaseolamin

作者：de Gouveia Neire Moura; Alves Fernanda Vieira; Furtado Fabiana Barcelos; Scherer Danielli Luana; Mundim Antonio Vicente; Espindola Foued Salmen^*

来源：Journal of Medicinal Food, 2014, 17(8): 915-920.

DOI：10.1089/jmf.2013.0044

• carbohydrate blockers
• diabetes
• Phaseolus
• serum biochemistry
• streptozotocin

We evaluated the polypeptide profiles, inhibition of human salivary alpha-amylase activity, and hemagglutination properties of a commercial phaseolamin sample. We also performed an in vivo assay to investigate the effects of a commercial phaseolamin treatment (100, 500, or 1500 mg/kg) over 20 days on the glycemia, body weight, and serum biochemical parameters (total cholesterol, triglycerides, alanine aminotransferase, and aspartate aminotransferase) of nondiabetic and streptozotocin-induced diabetic rats. The in vitro evaluation showed defined protein profiles, low hemagglutination activity, and high alpha-amylase inhibition. None of the experimental groups treated with phaseolamin or acarbose showed decreases in body weight. Our data demonstrate that phaseolamin inhibits amylase activity in vitro, reduces blood glucose levels, decreases or attenuates some of the renal and hepatic effects of diabetes in streptozotocin-induced rats, and could therefore have therapeutic potential in the treatment or prevention of the complications of diabetes.

• 出版日期2014-8