Detection of Betaproteobacteria inside the Mycelium of the Fungus Mortierella elongata

作者：Sato Yoshinori; Narisawa Kazuhiko; Tsuruta Kazuto; Umezu Masafumi; Nishizawa Tomoyasu; Tanaka Kenji; Yamaguchi Kaoru; Komatsuzaki Masakazu; Ohta Hiroyuki^*

来源：Microbes and Environments, 2010, 25(4): 321-324.

DOI：10.1264/jsme2.ME10134

• endobacterium
• N(2)O production
• Mortierella elongata
• soil fungus

Microscopic and molecular analyses showed the presence of endobacteria inside the mycelia of four out of twelve nitrous oxide (N(2)O)-producing fungal isolates identified as Mortierella elongata. The 16S rRNA gene was successfully amplified with DNA extracted directly from the endobacterium-containing fungal strains and all sequences were related to that of Candidatus Glomeribacter gigasporarum in the family Burkholderiaceae. Bacterial endotoxin was detected in the endobacterium-positive fungal strains but only trace levels were found in endobacterium-negative strains. No significant relationship was found between the fungal N(2)O-producing activity and the presence of endobacteria.

• 出版日期2010-12-3