High diversity of beta-lactamases in the General Hospital Vienna verified by whole genome sequencing and statistical analysis

作者：Barisic Ivan^*; Mitteregger Dieter; Hirschl Alexander M; Noehammer Christa; Wiesinger Mayr Herbert

来源：Infection, Genetics and Evolution, 2014, 27: 408-417.

DOI：10.1016/j.meegid.2014.08.014

• Carbapenemase
• ESBL
• AmpC
• Rarefaction curve
• Whole genome sequencing
• Chao1

The detailed analysis of antibiotic resistance mechanisms is essential for understanding the underlying evolutionary processes, the implementation of appropriate intervention strategies and to guarantee efficient treatment options. In the present study, 110 beta-lactam-resistant, clinical isolates of Enterobacteriaceae sampled in 2011 in one of Europe%26apos;s largest hospitals, the General Hospital Vienna, were screened for the presence of 31 beta-lactamase genes. Twenty of those isolates were selected for whole genome sequencing (WGS). In addition, the number of beta-lactamase genes was estimated using biostatistical models. %26lt;br%26gt;The carbapenemase genes bla(KPC-2), bla(KPC-3), and bla(VIM-4) were identified in carbapenem-resistant and intermediate susceptible isolates, bla(OXA-72) in an extended-spectrum beta-lactamase (ESBL)-positive one. Furthermore, the observed high prevalence of the acquired bla(DHA-1) and bla(CMY) AmpC beta-lactamase genes (70%) in phenotypically AmpC-positive isolates is alarming due to their capability to become carbapenem-resistant upon changes in membrane permeability. The statistical analyses revealed that approximately 55% of all beta-lactamase genes present in the General Hospital Vienna were detected by this study. In summary, this work gives a very detailed picture on the disseminated beta-lactamases and other resistance genes in one of Europe%26apos;s largest hospitals.

• 出版日期2014-10