A transcriptome screen in yeast identifies a novel assembly factor for the mitochondrial complex III

作者：Mathieu Lise; Marsy Sophie; Saint Georges Yann; Jacq Claude; Dujardin Genevieve^*

来源：Mitochondrion, 2011, 11(3): 391-396.

DOI：10.1016/j.mito.2010.12.002

• Yeast
• Respiratory complex biogenesis
• Complex III assembly
• mRNA localization

Starting from a transcriptome based study of the spatio-temporal expression of yeast genes encoding mitochondrial proteins of unknown function, we have identified the gene BCA1 (YLR077W). A FISH analysis showed that the BCA1 mRNA co-localized with the mitochondrial network. Cellular fractionation revealed that Bca1 is bound to the mitochondrial inner-membrane and protrudes into the inter-membrane space. We show that Bca1 controls an early step in complex III assembly and that the supra-molecular organization of Bca1 is dependent upon the assembly level of complex III. Thus, Bca1 is a novel assembly factor for the respiratory complex III.

• 出版日期2011-5