Advanced glycation endproducts regulate smooth muscle cells calcification in cultured HSMCs

作者：He, Hu-Qiang; Liu, Yong; Zeng, Hong; Sun, Xiao-Lei; Zhang, Lei; Zhang, Xue-Lin; Liao, Wen-Jun; Zhou, Xiang-Yu; He, Yan-Zheng^*

来源：International Journal of Clinical and Experimental Pathology, 2015, 8(10): 12260-12267.

• HSMCs
• calcification
• AGEs
• RAGE
• beta-catenin
• OPG

Objective: To investigate the mechanism of Advanced glycation end products (AGEs) promoting the calcification of smooth muscle cells. Methods: The successfully cultured smooth muscle cells were divided into three groups: normal culture group (group A), calcified culture group (group B), calcification + AGEs group (group C); the concentration of intracellular calcium ion was detected in each group; the promotion of AGEs on the calcification of HSMCs was confirmed by VON KOSSA staining; and the expressions of beta-catenin, RAGE, beta-catenin, OPG and E-cadherin protein were detected by immunofluorescence and western blot. Results: The morphology of the cells in each group showed that the amount of calcified plaques in calcification + AGES group were significantly higher than the calcification group. VON KOSSA staining showed that with increasing concentrations of AGE-BSA, the amount of its calcification gradually increased. Calcium concentration in Calcification + 20 mg/L AGEs group was significantly higher, followed by 40 mg/L AGEs group. The expression of beta-catenin increased with the increasing concentrations of AGEs. Conclusion: AGEs can promote the calcification of human femoral artery smooth muscle cells, with a concentration gradient effect. With increasing concentrations of AGEs, the expression of RAGE increased, indicating that AGEs-induced HSMCs proliferation was correlated with RAGE expression.

• 出版日期2015
• 单位西南医科大学