Adherens junctions and desmosomes are damaged by Entamoeba histolytica: Participation of EhCPADH complex and EhCP112 protease

作者：Hernandez Nava Elizabeth; Cuellar Patricia; Nava Porfirio; Chavez Munguia Bibiana; Schnoor Michael; Orozco Esther^*; Betanzos Abigail^*

来源：Cellular Microbiology, 2017, 19(11): e12761.

DOI：10.1111/cmi.12761

• Entamoeba histolytica
• adherens junctions
• desmosomes
• EhCPADH complex
• EhCP112
• EhADH
• epithelial invasion
• MDCK cells
• intestine
• murine amoebiasis model
• beta-catenin
• paracellular pathway

Entamoeba histolytica trophozoites adhere to epithelium at the cell-cell contact and perturb tight junctions disturbing the transepithelial electrical resistance. Behind tight junctions are the adherens junctions (AJs) that reinforce them and the desmosomes (DSMs) that maintain the epithelium integrity. The damage produced to AJs and DMSs by this parasite is unknown. Here, we studied the effect of the trophozoites, the EhCPADH complex, and the EhCP112 recombinant enzyme (rEhCP112) on AJ and DSM proteins. We found that trophozoites degraded beta-cat, E-cad, Dsp I/II, and Dsg-2 with the participation of EhCPADH and EhCP112. After contact of epithelial cells with trophozoites, immunofluorescence and transmission electron microscopy assays revealed EhCPADH and rEhCP112 at the intercellular space where they colocalised with beta-cat, E-cad, Dsp I/II, and Dsg-2. Moreover, our results suggested that rEhCP112 could be internalised by caveolae and clathrin-coated vesicles. Immunoprecipitation assays showed the interaction of EhCPADH with beta-cat and Dsp I/II. Besides, in vivo assays demonstrated that rEhCP112 concentrates at the cellular borders of the mouse intestine degrading E-cad and Dsp I/II. Our research gives the first clues on the trophozoite attack to AJs and DSMs and point out the role of the EhCPADH and EhCP112 in the multifactorial event of trophozoites virulence.

• 出版日期2017-11