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摘要
Centella asiatica (L.) Urban is a traditional herbal medicine used in Asiatic countries, and is commonly used to treat various wounds, leprosy, tuberculosis and lupus diseases. In this work, a new method based on microwave assisted extraction followed by liquid chromatography-diode array detection-electrospray ionization multistage tandem mass spectrometry analysis has been developed for the identification and quantification of biologically active constituents in C. asiatica, including asiatic acid, asiaticoside and madecassoside. The separation was performed on an Agilent Eclipse C-18 column (150 mm x 4.6 mm i.d., 5 mu m) with gradient elution of acetonitrile and 0.1% aqueous acetic acid within 50 min. Detection was performed at 205 nm. The calibration curves showed good linearity (r (2) > 0.9992). The limits of detection ranged from 1.2 to 1.6 mu g mL(-1). The intra- and inter-day precision was less than 3% and the recovery of the assay was in the range of 95.4-106.8%. The method was successfully applied to the quantification of the three constituents in different samples of C. asiatica. The results indicated that the developed method could be considered to be a simple, rapid and reliable method for the quality evaluation of C. asiatica. The samples were also analyzed on a liquid chromatography-electrospray ionization-time-of-flight mass spectrometry system to confirm the identification results.
- 出版日期2009-8
- 单位温州大学; 国家海洋局第一海洋研究所; 浙江工商大学
