Development of Quantum Dot Submicrobeads-based Fluorescent Immunochromatographic Test Strip for Rapid Detection of Chloramphenicol

作者：Ding Qiao-Qi; Li Li; Fan Wen-Tao; Lyu Ya-Nan; Hu Jian-Hua; Yan Li-Ping^*; Song Su-Quan^*

来源：Chinese Journal of Analytical Chemistry, 2017, 45(11): 1686-1693.

DOI：10.11895/j.issn.0253-3820.170300

• Chloramphenicol
• Quantum dot submicrobeads
• Immunochromatographic test strip

A fluoresc(e)nt immunochromatographic test strip based on the quantum dots submicrobeads (QBs) was developed for quantitative detection of chloramphenicol (CAP). In this method, monoclonal antibody of CAP and OBs complex fluorescent probe was first prepared using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide coupling approach, then complete antigen CAP-HS-BSA was synthesized and sprayed on nitrocellulose membrane as test line (T line). Similarly, goat anti-mouse antibody was sprayed as control line (C line). The time required for the analysis was 15 min, and the limit of detection (LOD) for CAP was 0. 1 mu g/L, with a working range of 0. 1 - 100 mu g/L. In spiked milk samples, the test strip demonstrated high recoveries in the range from 93. 3% to 97. 9% with relative standard deviations of less than 7%.

• 出版日期2017-11
• 单位南京农业大学