A novel isolated Terrabacter-like bacterium can mineralize 2-aminopyrimidine, the principal metabolite of microbial sulfadiazine degradation

作者：Tappe Wolfgang^*; Hofmann Diana; Disko Ulrich; Koeppchen Stephan; Kummer Sirgit; Vereecken Harry

来源：Biodegradation, 2015, 26(2): 139-150.

DOI：10.1007/s10532-015-9722-9

• Sulfonamides
• Sulfadiazine
• 2-Aminopyrimidine
• Degradation
• Metabolite
• Mineralization

Recently we showed that during the degradation of sulfadiazine (SDZ) by Microbacterium lacus strain SDZm4 the principal metabolite 2-aminopyrimidine (2-AP) accumulated to the same molar amount in the culture as SDZ disappeared (Tappe et al. Appl Environ Microbiol 79:2572-2577, 2013). Although 2-AP is considered a recalcitrant agent, long-term lysimeter experiments with C-14-pyrimidine labeled SDZ ([C-14](pyr)SDZ) provided indications for substantial degradation of the pyrimidine moiety of the SDZ molecule. Therefore, we aimed to enrich 2-AP degrading bacteria and isolated a pure culture of a Terrabacter-like bacterium, denoted strain 2APm3. When provided with C-14-labeled SDZ, M. lacus strain SDZm4 degraded [C-14](pyr)SDZ to [C-14]2-AP. Resting cells of 2APm3 at a concentration of 5 x 10(6) cells ml(-1) degraded 62 A mu M [C-14]2-AP to below the detection limit (0.6 A mu M) within 5 days. Disappearance of 2-AP resulted in the production of at least two transformation products (M1 and M2) with M2 being identified as 2-amino-4-hydroxypyrimidine. After 36 days, the transformation products disappeared and 83 % of the applied [C-14]2-AP radioactivity was trapped as (CO2)-C-14. From this we conclude that a consortium of two species should be able to almost completely degrade SDZ in soils.

• 出版日期2015-4