High-dynamic-range microscope imaging based on exposure bracketing in full-field optical coherence tomography

作者：Leong Hoi Audrey^*; Montgomery Paul C; Serio Bruno; Twardowski Patrice; Uhring Wilfried

来源：Optics Letters, 2016, 41(7): 1313-1316.

DOI：10.1364/OL.41.001313

By applying the proposed high-dynamic-range (HDR) technique based on exposure bracketing, we demonstrate a meaningful reduction in the spatial noise in image frames acquired with a CCD camera so as to improve the fringe contrast in full-field optical coherence tomography (FF-OCT). This new signal processing method thus allows improved probing within transparent or semitransparent samples. The proposed method is demonstrated on 3 mu m thick transparent polymer films of Mylar, which, due to their transparency, produce low contrast fringe patterns in white-light interference microscopy. High-resolution tomographic analysis is performed using the technique. After performing appropriate signal processing, resulting XZ sections are observed. Submicrometer-sized defects can be lost in the noise that is present in the CCD images. With the proposed method, we show that by increasing the signal-to-noise ratio of the images, submicrometer-sized defect structures can thus be detected.

• 出版日期2016-4-1