Nucleotide sequence variability in exon 4 of prolactin locus in Murrah buffaloes (Bubalus bubalis)

作者：Archana Verma^*

来源：Research Journal of Biotechnology, 2013, 8(6): 61-65.

• Prolactin
• exon 4
• PCR-RFLP
• PCR-SSCP
• Murrah buffalo

Prolactin plays an important role in mammary gland development, milk secretion and expression of milk protein genes. This is a candidate gene and a potential genetic marker for production traits in dairy animals. Therefore, present study aimed to screen buffalo herd maintained at National Dairy Research Institute, Karnal for the genetic polymorphism of the PRL gene using PCR-RFLP and PCR-SSCP analysis for future use of genetic variants as markers for animal selection. Genomic DNA was isolated from 150 lactating Murrah buffaloes maintained at National Dairy Research Institute, Karnal. Polymerase chain reaction (PCR) was set up to amplify exon 4 of prolactin locus using specific primers. PCR-RFLP analysis was carried out by digestion of 294 bp amplicon with Rsa I restriction enzyme.
The animals included in the study exhibited monomorphism. PCR-SSCP resulted in two band patterns. DNA sequencing revealed nucleotide sequence variation at a total of six positions: two in intron 3, three in exon 4 and one in intron 4 regions. All were silent mutations except G8477T resulting in amino acid substitution from glycine to cysteine at 53rd position. Identification of a specific DNA marker and its association with production traits may be utilized in developing specific breeding programmes for animal selection.

• 出版日期2013-6