Ubiquitin E3 Ligase A20 Facilitates Processing Microbial Product in Nasal Epithelial Cells

作者：An, Yun-Fang; Li, Tong-Li; Geng, Xiao-Rui; Yang, Gui; Zhao, Chang-Qing^*; Yang, Ping-Chang

来源：JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287(42): 35318-35323.

DOI：10.1074/jbc.M112.392639

• TRANSEPITHELIAL ANTIGEN TRANSPORT
• STAPHYLOCOCCUS-AUREUS COLONIZATION
• ENTEROTOXIN-B
• SENSITIZATION
• IGE

Microbial products play a role in the pathogenesis of allergic diseases; ubiquitin E3 ligase A20 (A20) is an important molecule in regulating inflammation in the body. The present study aims to elucidate the role of A20 in processing the absorbed microbial products in nasal epithelial cells. Human nasal mucosal specimens were collected from patients with or without chronic rhinitis and analyzed by immunohistochemistry. Human nasal epithelial cell line, RPMI2650 cell, was employed to assess the role of A20 in processing the absorbed staphylococcal enterotoxin B (SEB). The RPMI2650 cells absorbed SEB in the culture. The increase in A20 was observed in RPMI2650 cells in parallel to the absorption of SEB. A20 is a critical molecule in the degradation of SEB in the nasal epithelial cells by promoting the tethering of endosomes and lysosomes. A20 plays a critical role in processing of the absorbed SEB in nasal epithelial cells.

• 出版日期2012-10-12
• 单位山西医科大学第二医院; 山西省人民医院