Ambient particulate matter activates the aryl hydrocarbon receptor in dendritic cells and enhances Th17 polarization

作者：Castaneda Alejandro R; Pinkerton Kent E; Bein Keith J; Magana Mendez Alfonso; Yang Houa T; Ashwood Paul; Vogel Christoph F A^*

来源：Toxicology Letters, 2018, 292: 85-96.

DOI：10.1016/j.toxlet.2018.04.020

• Aryl hydrocarbon receptor (AhR)
• Dendritic cells (DCs)
• Particulate matter (PM)
• Polycyclic aromatic hydrocarbons (PAHs)
• Th17 cells

The objective of this study was to explore the role of the aryl hydrocarbon receptor (AhR) in ambient particulate matter (PM)-mediated activation of dendritic cells (DCs) and Th17-immune responses in vitro. To assess the potential role of the AhR in PM-mediated activation of DCs, co-stimulation, and cytokine expression, bone marrow (BM)-derived macrophages and DCs from C57BL/6 wildtype or AhR knockout (AhR(-/-)) mice were treated with PM. Th17 differentiation was assessed via co-cultures of wildtype or AhR(-/-) BMDCs with autologous naive T cells. PM2.5 significantly induced AhR DNA binding activity to dioxin responsive elements (DRE) and expression of the AhR repressor (AhRR), cytochrome P450 (CYP) 1A1, and CYP1B1, indicating activation of the AhR. In activated (OVA sensitized) BMDCs, PM2.5 induced interleukin (IL)-1 beta, CD80, CD86, and MHC class II, suggesting enhanced DC activation, co-stimulation, and antigen presentation; responses that were abolished in AhR deficient DCs. DC-T cell co-cultures treated with PM and lipopolysaccharide (LPS) led to elevated IL-17A and IL-22 expression at the mRNA level, which is mediated by the AhR. PM-treated DCs were essential in

• 出版日期2018-8