A Facile Preparation Process of Magnetic Aldehyde-Functionalized Ni-0.Zn-5(0).5Fe2O4@SiO2 Nanocomposites for Immobilization of Penicillin G Acylase (PGA)

作者：Liu, Ruijiang^*; Chen, Dan; Fu, Hongxia; Lv, Pingxue; Zhang, Die; He, Yunjie

来源：Journal of Nanoscience and Nanotechnology, 2017, 17(2): 893-899.

DOI：10.1166/jnn.2017.12694

• Ni0.5Zn0.5Fe2O4@SiO2
• Penicillin G Acylase
• Immobilization

A facile sol combustion and gel calcination process has been reported for the preparation of coreshell magnetic Ni0.5Zn0.5Fe2O4@SiO2 nanocomposites. The morphology, chemical composition, structure and magnetic property of as-prepared nanocomposites were investigated by XRD, VSM, BET, SEM, and TEM, and the magnetic Ni0.5Zn0.5Fe2O4@SiO2 nanocomposites were characterized with average size of about 25 nm, saturation magnetization of 90.8 Am-2/kg and the specific surface area of 67.1 m(2)/g. The surface of Ni0.5Zn0.5Fe2O4@SiO2 nanocomposites was functionalized with glutaraldehyde to form the aldehyde-functionalized magnetic Ni0.5Zn0.5Fe2O4@SiO2 nanocomposites, and penicillin G acylase (PGA) was successfully immobilized onto them. And the immobilized PGA exhibited high effective activity, good stability of enzyme catalyst and good reusability, and could retain 63.5% of initial activity after 12 consecutive operations. The kinetic parameters were determined, and the value of K-m for the immobilized PGA (161.7 mmol/L) is higher than that of the free PGA (3.5 mmol/L), while vmax (1.626 mmol/min) is also larger than that of the free PGA (0.838 mmol/min), which revealed that the immobilization of PGA onto Ni0.5Zn0.5Fe2O4@SiO2 nanocomposites was an efficient and simple way for preparation of stable PGA.

• 出版日期2017-2
• 单位江苏大学