Antibacterial constituents of Fructus Chebulae Immaturus and their mechanisms of action

作者：Li, Kun; Lin, Yue; Li, Bin; Pan, Taowen; Wang, Fei; Yuan, Ruqiang; Ji, Jianjun; Diao, Yunpeng^*; Wang, Shouyu^*

来源：BMC Complementary and Alternative Medicine, 2016, 16(1): 183.

DOI：10.1186/s12906-016-1162-5

• Fructus Chebulae Immaturus
• Total tannin
• Ethyl gallate
• Antibacterial
• Electron microscopy

Background: To extract, purify, and identify the effective constituents of aqueous extract of Fructus Chebulae Immaturus, and analyze the bactericidal effects of total tannins.
Methods: Preparative thin layer chromatography and semi-preparative high performance liquid chromatography were used to isolate and purify the total tannin fraction. H-1- and C-13-NMR spectroscopy were used to elucidate compound structures. The antibacterial activities of total tannins and ethyl gallate on Klebsiella pneumoniae (KP) and Staphylococcus aureus (SA) were determined through minimum inhibitory concentration and minimum bactericidal concentration assays. Their antibacterial mechanisms of action were explored by transmission electron microscopy and scanning electron microscopy.
Results: Five compounds were isolated: ellagic acid, ethyl gallate, arjugenin, beta-sitosterol, and tri-n-butyl chebulate. Tri-n-butyl chebulate is a newly-reported compound. Total tannins and ethyl gallate both had favorable bactericidal effects against KP and SA.
Conclusion: In vivo and in vitro pharmacodynamic experiment demonstrated that the effective components of Fructus Chebulae Immaturus possessed significant antibacterial effects, and were nontoxic and safe.

• 出版日期2016-7-2
• 单位辽宁中医药大学; 辽宁师范大学; 大连医科大学