A stem cell medium containing neural stimulating factor induces a pancreatic cancer stem-like cell-enriched population

作者：Watanabe Yusaku; Yoshimura Kiyoshi; Yoshikawa Koichi; Tsunedomi Ryoichi; Shindo Yoshitaro; Matsukuma Sou; Maeda Noriko; Kanekiyo Shinsuke; Suzuki Nobuaki; Kuramasu Atsuo; Sonoda Kouhei; Tamada Koji; Kobayashi Sei; Saya Hideyuki; Hazama Shoichi; Oka Masaaki^*

来源：International Journal of Oncology, 2014, 45(5): 1857-1866.

DOI：10.3892/ijo.2014.2603

• cancer stem cells
• cell culture method
• pancreatic cancer

Cancer stem cells (CSCs) have been studied for their self-renewal capacity and pluripotency, as well as their resistance to anticancer therapy and their ability to metastasize to distant organs. CSCs are difficult to study because their population is quite low in tumor specimens. To overcome this problem, we established a culture method to induce a pancreatic cancer stem-like cell (P-CSLC)-enriched population from human pancreatic cancer cell lines. Human pancreatic cancer cell lines established at our department were cultured in CSC-inducing media containing epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), leukemia inhibitory factor (LIF), neural cell survivor factor-1 (NSF-1), and N-acetylcysteine. Sphere cells were obtained and then transferred to a laminin-coated dish and cultured for approximately two months. The surface markers, gene expression, aldehyde dehydrogenase (ALDH) activity, cell cycle, and tumorigenicity of these induced cells were examined for their stem cell-like characteristics. The population of these induced cells expanded within a few months. The ratio of CD24high, CD44high, epithelial specific antigen (ESA) high, and CD44variant (CD44v) high cells in the induced cells was greatly enriched. The induced cells stayed in the GO/G1 phase and demonstrated mesenchymal and sternness properties. The induced cells had high tumorigenic potential. Thus, we established a culture method to induce a P-CSLC-enriched population from human pancreatic cancer cell lines. The CSLC population was enriched approximately 100-fold with this method. Our culture method may contribute to the precise analysis of CSCs and thus support the establishment of CSC-targeting therapy.

• 出版日期2014-11