An animal model of preclinical diagnosis of pancreatic ductal adenocarcinomas

作者：Fukamachi Katsumi; Tanaka Hajime; Hagiwara Yoshiaki; Ohara Hirotaka; Joh Takashi; Iigo Masaaki; Alexander David B; Xu Jiegou; Long Ne; Takigahira Misato; Yanagihara Kazuyoshi; Hino Okio; Saito Izumu; Tsuda Hiroyuki^*

来源：Biochemical and Biophysical Research Communications, 2009, 390(3): 636-641.

DOI：10.1016/j.bbrc.2009.10.019

• Pancreas cancer
• Serum marker
• Erc
• Mesothelin
• Animal model

Pancreatic ductal adenocarcinoma (PDA) is a highly lethal disease, which is usually diagnosed in an advanced stage. Animal PDA models which reflect the human condition are clearly necessary to develop early diagnostic tools and explore new therapeutic approaches. We have established transgenic rats carrying a mutated H- or K-ras gene (Hras250 and Kras327) controlled by Cre/loxP activation. These animals develop PDA which are histopathologically similar to that in humans. We utilized this model to identify biomarkers to detect early PDA. We report here that serum levels of Erc/Mesothelin are significantly higher in rats bearing PDA than in controls. Importantly, the levels are significantly elevated in rats before grossly visible carcinomas develop. Even in rats with very small microscopic ductal carcinoma lesions, elevated serum Erc/Mesothelin can be detected. We believe this is the first report of a pancreas tumor animal model in which pre-symptomatic lesions can be diagnosed.

• 出版日期2009-12-18
• 单位河北医科大学