An antifungal peptide from Phaseolus vulgaris cv. brown kidney bean

作者：Chan Yau Sang; Wong Jack Ho; Fang Evandro Fei; Pan Wen Liang; Ng Tzi Bun^*

来源：Acta Biochimica et Biophysica Sinica, 2012, 44(4): 307-315.

DOI：10.1093/abbs/gms003

• isolation
• antifungal peptide
• defensin-like
• Phaseolus vulgaris

A 5.4-kDa antifungal peptide, with an N-terminal sequence highly homologous to defensins and inhibitory activity against Mycosphaerella arachidicola (IC50 3 M), Setospaeria turcica and Bipolaris maydis, was isolated from the seeds of Phaseolus vulgaris cv. brown kidney bean. The peptide was purified by employing a protocol that entailed adsorption on Affi-gel blue gel and Mono S and finally gel filtration on Superdex 75. The antifungal activity of the peptide against M. arachidicola was stable in the pH range 312 and in the temperature range 0C to 80C. There was a slight reduction of the antifungal activity at pH 2 and 13, and the activity was indiscernible at pH 0, 1, and 14. The activity at 90C and 100C was slightly diminished. Deposition of Congo red at the hyphal tips of M. arachidicola was induced by the peptide indicating inhibition of hyphal growth. The lack of antiproliferative activity of brown kidney bean antifungal peptide toward tumor cells, in contrast to the presence of such activity of other antifungal peptides, indicates that different domains are responsible for the antifungal and antiproliferative activities.

• 出版日期2012-4
• 单位香港中文大学