A Fluorescent-Probe Detecting CYP2C9*3 DNA

作者：Li Qing yong^*; Qu Zhen huan; Zu Yuan gang; Fu Yu jie

来源：Spectroscopy and Spectral Analysis, 2010, 30(6): 1520-1524.

DOI：10.3964/j.issn.1000-0593(2010)06-1520-05

• Fluorescent-probe
• Detection
• CYP2C9*3 DNA
• Polymorphism detection

The mismatched CYP2C9 * 3 DNA was detected by a exciplex fluorescent-probe system. The exciplex fluorescentprobe model system comprises of two 12-mer oligonucleotides fluorescent-probes, complementary to neighbouring sites of a 24 (47) -mer DNA target and plasmid target, each equipped with moieties able to form an exciplex on correct, contiguous hybridization. Very similar results were obtained between the 24-mer target and the 47-mer target when WT and MT systems were detected by the exciplex fluorescent-probe. Exciplex bands at 505 nm were found for both 24(47)-mer WT and 24(47)-mer MT-targets at 5 degrees C but were not distinctive enough to distinguish 24(47)-mer WT-target and 24(47)-mer MT-target. However the experiments were carried out at Tm, the exciplex band disappeared almost completely for 24 (47)-mer MT-target system like control system, and there was still a strong exciplex band for the 24(47)-mer WT target system. Exciplex peaks at 505 nm were seen for the WT circular plasmids system, but not for MT circular plasmids. Therefore, mismatches of CYP2C9* 3 DNA can be effectively detected by this exciplex construct, giving potential for single nucleotide polymorphism detection.

• 出版日期2010-6
• 单位东北林业大学