Neuroprotective Effects of Carpinus tschonoskii MAX on 6-Hydroxydopamine-Induced Death of PC12 Cells

作者：Kim Min Kyoung; Kim Sang Cheol; Kang Jung Il; Boo Hye Jin; Hyun Jin Won; Koh Young Sang; Park Deok Bae; Yoo Eun Sook; Kang Ji Hoon; Kang Hee Kyoung^*

来源：Biomolecules & Therapeutics, 2010, 18(4): 454-462.

DOI：10.4062/biomolther.2010.18.4.454

• Carpinus tschonoskii MAX
• 6-OHDA
• PC12 cells
• Apoptosis
• MEF2D
• Akt

The present study investigated the neuroprotective effect of Carpinus tschonoskii MAX and its intracellular protective mechanism on 6-hydroxydopamine (6-OHDA)-induced oxidative damage in PC12 cells. We found that pretreatment of PC12 cells with C. tschonoskii extract significantly inhibited the cell death induced by 6-OHDA in a dose dependent manner. C. tschonoskii extract decreased 6-OHDA-induced apoptotic events such as chromatin condensation, DNA fragmentation, the decrease of Bcl-2/Bax ratio, caspase-3 activation and PARP cleavage. C. tschonoskii extract also reduced generation of 6-OHDA-induced reactive oxygen species and nitric oxide. Furthermore, C. tschonoskii extract up-regulated the myocyte enhancer factor 2 D (MEF2D), a critical transcription factor for neuronal survival, and Akt activity, whereas it inhibited the activity of ERK1/2 and JNK. The results suggest that C. tschonoskii extract decreases 6-OHDA-induced oxidative stress and could prevent PC12 cell apoptosis induced by 6-OHDA via the up-regulation of MEF2D and Akt activity, and thus may have application in developing therapeutic agents for Parkinson's disease.

• 出版日期2010-10-31
• 单位河北医科大学