Mild Oxidative Stress Induces Redistribution of BACE1 in Non-Apoptotic Conditions and Promotes the Amyloidogenic Processing of Alzheimer's Disease Amyloid Precursor Protein

作者：Tan, Jiang-Li; Li, Qiao-Xin; Ciccotosto, Giuseppe D.; Crouch, Peter John; Culvenor, Janetta Gladys; White, Anthony Robert; Evin, Genevieve^*

来源：PLos One, 2013, 8(4): e61246.

DOI：10.1371/journal.pone.0061246

• BETA-SECRETASE ACTIVITY
• FACTOR-KAPPA-B
• A-BETA
• UP-REGULATION
• DIFFERENTIAL REGULATION
• REDOX REGULATION
• GENE-EXPRESSION
• GAMMA-SECRETASE
• SORTING SIGNAL
• APP

BACE1 is responsible for beta-secretase cleavage of the amyloid precursor protein (APP), which represents the first step in the production of amyloid beta (A beta) peptides. Previous reports, by us and others, have indicated that the levels of BACE1 protein and activity are increased in the brain cortex of patients with Alzheimer's disease (AD). The association between oxidative stress (OS) and AD has prompted investigations that support the potentiation of BACE1 expression and enzymatic activity by OS. Here, we have established conditions to analyse the effects of mild, non-lethal OS on BACE1 in primary neuronal cultures, independently from apoptotic mechanisms that were shown to impair BACE1 turnover. Six-hour treatment of mouse primary cortical cells with 10-40 mu M hydrogen peroxide did not significantly compromise cell viability but it did produce mild oxidative stress (mOS), as shown by the increased levels of reactive radical species and activation of p38 stress kinase. The endogenous levels of BACE1 mRNA and protein were not significantly altered in these conditions, whereas a toxic H2O2 concentration (100 mu M) caused an increase in BACE1 protein levels. Notably, mOS conditions resulted in increased levels of the BACE1 C-terminal cleavage product of APP, beta-CTF. Subcellular fractionation techniques showed that mOS caused a major rearrangement of BACE1 localization from light to denser fractions, resulting in an increased distribution of BACE1 in fractions containing APP and markers for trans-Golgi network and early endosomes. Collectively, these data demonstrate that mOS does not modify BACE1 expression but alters BACE1 subcellular compartmentalization to favour the amyloidogenic processing of APP, and thus offer new insight in the early molecular events of AD pathogenesis.

• 出版日期2013-4-17