BODIPY/Nile-Red-Based Efficient FRET Pair: Selective Assay of Endoplasmic Reticulum Membrane Fluidity

作者：Yang, Zhigang; Wi, Youngjin; Yoon, Young-Min; Verwilst, Peter; Jang, Joo Hee; Kim, Tae Woo^*; Kang, Chulhun^*; Kim, Jong Seung^*

来源：Chemistry-An Asian Journal, 2016, 11(4): 527-531.

DOI：10.1002/asia.201501060

• endoplasmic reticulum
• fluorescence
• FRET
• membrane fluidity
• metabolic syndrome

We synthesized a boron-dipyrromethene (BODIPY)/Nile Red hybrid probe capable of selectively recognizing fluidity changes in the endoplasmic reticulum (ER) membrane due to its preferential localization to the ER and strong energy transfer from BODIPY to the Nile Red moiety, emitting only in nonaqueous environments. ER membrane fluidity in HepG2 cells was markedly reduced by a cell model of metabolic syndrome.

• 出版日期2016-2-18
• 单位深圳大学