Arabidopsis SWI/SNF chromatin remodeling complex binds both promoters and terminators to regulate gene expression

作者：Archacki Rafal^*; Yatusevich Ruslan; Buszewicz Daniel; Krzyczmonik Katarzyna; Patryn Jacek; Iwanicka Nowicka Roksana; Biecek Przemyslaw; Wilczynski Bartek; Koblowska Marta; Jerzmanowski Andrzej; Swiezewski Szymon^*

来源：Nucleic Acids Research, 2017, 45(6): 3116-3129.

DOI：10.1093/nar/gkw1273

ATP-dependent chromatin remodeling complexes are important regulators of gene expression in Eukaryotes. In plants, SWI/SNF-type complexes have been shown critical for transcriptional control of key developmental processes, growth and stress responses. To gain insight into mechanisms underlying these roles, we performed whole genome mapping of the SWI/SNF catalytic subunit BRM in Ara-bidopsis thaliana, combined with transcript profiling experiments. Our data showthat BRMoccupies thousands of sites in Arabidopsis genome, most of which located within or close to genes. Among identified direct BRM transcriptional targets almost equal numbers were up-and downregulated upon BRM depletion, suggesting that BRM can act as both activator and repressor of gene expression. Interestingly, in addition to genes showing canonical pattern of BRM enrichment near transcription start site, many other genes showed a transcription termination site-centred BRM occupancy profile. We found that BRM-bound 3' gene regions have promoter-like features, including presence of TATA boxes and high H3K4me3 levels, and possess high antisense transcriptional activity which is subjected to both activation and repression by SWI/SNF complex. Our data suggest that binding to gene terminators and controlling transcription of non-coding RNAs is anotherway through which SWI/SNF complex regulates expression of its targets.

• 出版日期2017-4-7