Development of a proteoliposome model to probe transmembrane electron-transfer reactions

作者:White Gaye F; Shi Zhi; Shi Liang; Dohnalkova Alice C; Fredrickson James K; Zachara John M; Butt Julea N; Richardson David J; Clarke Thomas A*
来源:Biochemical Society Transactions, 2012, 40: 1257-1260.
DOI:10.1042/BST20120116

摘要

The mineral-respiring bacterium Shewanella oneidensis uses a protein complex, MtrCAB, composed of two decahaem cytochromes brought together inside a transmembrane porin to transport electrons across the outer membrane to a variety of mineral-based electron acceptors. A proteoliposome system has been developed that contains Methyl Viologen as an internalized electron carrier and valinomycin as a membrane-associated cation exchanger. These proteoliposomes can be used as a model system to investigate MtrCAB function.

  • 出版日期2012-12