A novel practical approach to ocular tissue preservation: a pilot project

作者：Rushton J O^*; Fuchs Baumgartinger A; Nell B

来源：Wiener Tierarztliche Monatsschrift, 2016, 103(5-6): 137-141.

• eye
• preservation
• swine
• vacuum

To date, preservation of ocular tissues for use as donor material represents a challenge to both human and veterinary ophthalmologists. Therefore the aim of the current study was to determine the potential benefits of vacuum preservation in ocular tissue storage. Nine porcine eyes, enucleated within one hour post mortem were used. Four eyes were vacuum packed using a commercially available vacuum device according to manufacturer's specifications. Each vacuum packed eye was stored at +20 degrees C [room temperature], +4 degrees C, -20 degrees C, or -80 degrees C. Four eyes were stored at the same temperatures with no prior vacuum preservation. The remaining eye was immediately placed in 10% formaldehyde as a control. All eyes were stored for seven days. Upon completion of the storage phase, all eyes were placed in 10% formaldehyde and submitted to histopathologic examination. The eyes were evaluated with special emphasis on tissue preservation and ranked accordingly (1=best; 9=worst). The eye which was vacuum packed and stored at -20 degrees C was best preserved, in comparison to the control eye. The results indicate that vacuum packaging may have beneficial effects on the preservation of entire globes.

• 出版日期2016