A Surface Plasmon Resonance Biochip That Operates Both in the Angular and Wavelength Interrogation Modes

作者：Oliveira Leiva Casemiro^*; Moreira Cleumar da Silva; Thirstrup Carsten; Kurt Melcher Elmar Uwe; Nogueira Lima Antonio Marcus; Neff Helmut

来源：IEEE Transactions on Instrumentation and Measurement, 2013, 62(5): 1223-1232.

DOI：10.1109/TIM.2012.2232433

• Biomedical transducers
• optical spectroscopy
• optical transducers
• optoelectronic devices
• plasmons
• thin-film devices

This paper presents a surface plasmon resonance system based on a polymer prism chip. The device allows operation in both the angular and wavelength interrogation modes. The biochip design is discussed emphasizing the effect of the ambient temperature over the optical behavior. Birefringence effect, biochip polishing, and responsivity are also reported. The basic mathematical formulation for both operating modes is discussed, and morphological parameters are considered in the data analysis. Experimental sensorgrams obtained at both interrogation modes with the same polymer prism chip are presented and compared. The experimental sensorgrams obtained with assays providing reversible (phosphate buffered saline and hypochlorite solutions) and irreversible (neutravindin solution) bindings demonstrate the feasibility of the proposed design.

• 出版日期2013-5