A Two-Step Protocol to Remove Endotoxins from Human-Like Collagen

作者：Zhang Jing; Zhu Chenhui^*; Fan Daidi; Ma Xiaoxuan; Mi Yu; Xue Wenjiao

来源：Separation Science and Technology (Philadelphia), 2015, 50(7): 993-1001.

DOI：10.1080/01496395.2014.978467

• human-like collagen
• response surface methodology
• affinity chromatography
• endotoxin removal
• Triton X-114 two-phase extraction

A two-step protocol to remove endotoxins from recombinant human-like collagen was investigated. To meet the clinical need, Triton X-114 two-phase extraction system was applied as the first step to remove large amounts of endotoxins, while affinity chromatography was used as the second step to achieve further purification. Under the best experimental condition optimized through response surface methodology techniques, endotoxin level was 0.025 similar to 0.25 EU/mg and protein recovery was 81.9%, respectively, which indicated that 99% of endotoxins were eliminated with high protein recovery. Therefore, the two-step protocol can be exploited as an efficient method for endotoxin elimination in biotechnology processes.

• 出版日期2015-5-3
• 单位西北大学