A Sterol Biosynthetic Gene AtCYP51A2 Promoter for Constitutive and Ectopic Expression of a Transgene in Plants

作者：Lee Hyoungseok; Oh Hyun Jeong; Ahn Hyo Min; Oh Chang Jae; Jeong Jin Ho; Jeon Gyeong Lyong; An Chung Sun; Choi Sang Bong; Kim Ho Bang^*

来源：Journal of Plant Biology, 2008, 51(5): 359-365.

DOI：10.1007/bf03036139

• Arabidopsis CYP51A2 promoter
• beta-glucuronidase
• binary vector
• CaMV 35S promoter
• constitutive overexpression
• sterol biosynthesis

Arabidopsis CYP51A2 (AtCYP51A2) mediates the sterol 14 alpha-demethylation step in de novo sterol biosynthesis, and is constitutively and highly expressed in all plant tissues (Kim et al., 2005). We exploited the molecular features of its expression and the fundamental role of sterol biosynthesis in cells to develop a plant-derived promoter. Our GUS expression analysis between transgenic Arabidopsis lines for AtCYP51A2::GUS and 35S::GUS revealed that activity of the AtCYP51A2 promoter was comparable to that of the 35S promoter, based on enzymatic activities and protein levels. The AtCYP51A2 promoter was also constitutively active in transgenic tobacco, indicating that 5' regulatory elements could be conserved among CYP51 promoters in dicot plants. A homologue of AtCYP51A2 was identified from rape seed, a crop species closely related to Arabidopsis. Its constitutive tissue expression pattern implies that the application of this AtCYP51A2 promoter is possible for that species. Based on these results, we present a new binary vector system with the plant-derived AtCYP51A2 promoter, which is able to constitutively and ectopically drive a transgene in various dicotyledonous plants.

• 出版日期2008-9-30
• 单位中国极地研究中心