Altered acetylcholinesterase levels in the spinal cord anterior horn and dorsal root ganglion following sciatic nerve ischemia

作者：Yang Zhenjun; Wang Pei; Yang Songhe; Xue Jingfeng^*

来源：Neural Regeneration Research, 2009, 4(1): 77-80.

• sciatic nerve
• ischemia
• acetylcholine esterase
• neuron

BACKGROUND: Peripheral nerve ischemia has been shown to result in ischemic fiber degeneration and axoplasmic transport disturbance. However, the effect on acetylcholinesterase (AChE) expression in relevant cells following sciatic nerve ischemia remains unclear.
OBJECTIVE: To observe AChE concentration changes following peripheral nerve ischemia.
DESIGN, TIME AND SETTING: The present comparative observation, neuroanatomical experiment was performed at the Central Laboratory Animal of Chengde Medical College between 2006 and 2007.
MATERIALS: A total of 20 healthy, adult, Wistar rats were randomized into two groups (n = 10): 8-day ischemia and 14-day ischemia.
METHODS: Ischemia injury was induced in the unilateral sciatic nerve (experimental side) through ligation of the common iliac artery. The contralateral side received no intervention, and served as the control side. Rats in the 8-day ischemia and 14-day ischemia groups were allowed to survive for 8 and 14 days, respectively.
MAIN OUTCOME MEASURES: The L(5) lumbar spinal cord and the L5 dorsal root ganglion were removed from both sides and sectioned utilizing a Leica vibrating slicer. AChE cellular expression was detected using Karnovsky-Root, and the number of AChE-positive cells and average gray value were analyzed using a MiVnt image analysis system.
RESULTS: In the 8-day ischemia group, AChE-positive cell numbers were significantly less in the dorsal root ganglion and spinal cord anterior horn of the experimental side, but the average gray value was significantly greater, compared with the control side (P < 0.05). These changes were more significant in the 14-day ischemia group than in the 8-day ischemia group (P < 0.01).
CONCLUSION: Peripheral nerve ischemia leads to decreased AChE expression in the associated cells in a time-dependent manner.

• 出版日期2009-1
• 单位承德医学院