Development of carvedilol assay in tablet dosage form using HPLC with fluorescence detection

作者：Sripalakit Pattana^*; Kaewnok Somsak; Tubtonglang Sakawrat

来源：Maejo International Journal of Science and Technology, 2010, 4(1): 8-19.

• carvedilol
• HPLC
• validation
• dissolution test
• quality control

A simple HPLC method was developed and validated for quantitation of carvedilol in dissolution medium and tablet dosage form. Chromatographic separation was achieved on a Alltima (R) C18 (250 mmx4.6 mm) column using a mobile phase containing 0.01 M Na(2)HPO(4) in water and acetonitrile (30:70 v/v) adjusted to pH 3.0 by orthophosphoric acid at a flow rate of 1.0 ml/min and employing fluorescence detection with 300- nm excitation and 343-nm emission wavelengths. The method was validated for specificity, linearity, accuracy, precision and stability. Dissolution test parameters were also investigated. Moreover, the proposed analytical method was applied to monitor the formulation content uniformity and labelled amount of commercially available carvedilol drugs.

• 出版日期2010-4