A recombinant human HLA-class I antigen linked to dextran elicits innate and adaptive immune responses

作者：Scholler Jorgen; Singh Mahavir; Bergmeier Lesley; Brunstedt Katja; Wang Yufei; Whittall Trevor; Rahman Durdana; Pido Lopez J; Lehner T^*

来源：Journal of Immunological Methods, 2010, 360(1-2): 1-9.

DOI：10.1016/j.jim.2010.05.008

• HSP
• Dendritic cells
• HIV
• SIV

The objective of this study was to produce and evaluate the immunogenic potential of a recombinant HLA-class I antigen linked to dextran. The HLA-A*0201 heavy chain and 132 microglobulin were cloned by PCR amplification of overlapping oligonucleotides and produced in E. coli. These were assembled with a CMV binding peptide motif, the HLA complex was biotinylated and bound by streptavidin coated dextran at a ratio of 24 HLA to 1 dextran molecule (termed Dextramer). Allostimulation of human PBMC in vitro and in vivo immunization of Balb c mice with the HLA-A*0201 construct elicited CD4(+) and CD8(+) T cell proliferative responses, IgG specific antibodies in mice and in human T cell proliferation and APOBEC3G mRNA. These adaptive and innate immune responses induced by a novel recombinant HLA construct in human cells and mice suggest their application as a potential vaccine candidate against HIV infection.

• 出版日期2010-8-31