Levels of Blood CD1c(+) mDC1 and CD1c(hi) mDC1 Subpopulation Reflect Disease Activity in Noninfectious Uveitis

作者：Chen Ping; Tucker William; Hannes Susan; Liu Baoying; Si Han; Gupta Ankur; Lee Richard W J; Sen H Nida; Nussenblatt Robert B^*

来源：Investigative Ophthalmology & Visual Science, 2015, 56(1): 346-351.

DOI：10.1167/iovs.14-15416

• CD1c(+) mDC1
• CD1c(hi) mDC1 subpopulation
• noninfectious uveitis
• autoimmune disease

PURPOSE. Myeloid dendritic cells (mDCs) play an important role in autoimmune diseases. However, the role of blood CD1c(+) myeloid dendritic cells 1 (mDC1s), the subset of human blood mDCs, is not well understood in noninfectious uveitis.
METHODS. Fresh peripheral blood samples from human noninfectious uveitis patients (n = 32) and healthy controls (HCs) (n = 64) were stained with FITC-Lineage 1 (Lin1), PERCP-HLADR, and PE-CD1c antibodies. The levels of mDC1 were quantified by using flow cytometric analysis. Longitudinal data from patients (n = 16) were analyzed to correlate the levels of mDC1 with disease activity.
RESULTS. Blood CD1c(+) mDC1 and its subpopulation, CD1c(hi) mDC1, were increased in uveitis patients compared with HCs. Longitudinal data demonstrated that both the CD1c(+) mDC1 and CD1c(hi) mDC1 subpopulation reflected a dynamic change in clinical uveitis activity: CD1c expression was increased in active uveitis but decreased when uveitis became inactive.
CONCLUSIONS. Given these observations, an alteration in blood CD1c(+) mDC1 and the CD1c(hi) mDC1 subpopulation could be a potential biomarker to monitor clinical uveitis activity within patients.

• 出版日期2015-1
• 单位NIH