Large-scale bone mineral histomorphometry - report of a simplified technique

作者：Karantzoulis Vasilis^*; Liapi Charis; Papaggelopoulos Panagiotis

来源：Histopathology, 2012, 61(6): 1174-1182.

DOI：10.1111/j.1365-2559.2012.04335.x

• epoxy resin
• silicon carbide
• surface-stained block grinding
• undecalcified bone
• von Kossa

Karantzoulis V, Liapi C %26 Papaggelopoulos P ?(2012) Histopathology?Large-scale bone mineral histomorphometry report of a simplified technique Aims: The aim of this study was the development of a simplified technique for bone mineral histomorphology on large undecalcified bone samples. Established techniques, such as undecalcified bone thin sectioning, ultrathin grinding, surface-stained block grinding and micro-computerized tomography (CT), are expensive, time-consuming and put very high demands on equipment, safety standards, personnel and laboratory facilities. Methods and results: The method is based on the surface-stained block-grinding principle; however, its novelty lies in the selection of user-friendly, safe and low-cost materials, equipment and digitization techniques. We describe in detail the relevant steps, as well as many practical tips for their successful implementation: accurate bone cutting in thin sections with a customized arrangement on a commercial bandsaw, defatting with sodium hypochlorite, embedding in epoxy resin blocks at room temperature, silicon carbide paper grinding, von Kossa staining, flatbed scanner digitization and image processing. Conclusion: We believe that the proposed methodology could contribute to the expansion of the study of bone tissue, as it enables the rapid examination of bone specimens on a large scale with minimal laboratory requirements and consumables costs.

• 出版日期2012-12